Biopolym. Cell. 1992; 8(2):44-49.
Cell Biology
Immunochemical identification of apoprotein A-I in the chromatin of rat hepatocyte nuclei
1Panin L. E., 1Polyakov L. M., 1Usynin I. Ph., 1Kuzmenko A. P., 1Poteryaeva O. N.
- Institute of Biochemistry Russian Academy of Medical Sciences
Str. Timakov 2, Novosibirsk, Russian Federation, 630117
Abstract
Two proteins of 28000 and 14000 immunologically identifical to apoprotein A-I were found in the chromatin of rat hepatocyte nuclei of using immunoblotting and enzyme-linked immunosorbent assay. The 28 000 protein was present in the trans-criptionally active chromatin and in the nuclear matrix. The 14000 protein was present in the repressed chromatin. The molecular weights of these protein determinated by SDS-PAGE, and immunochemical identity allow us to propose, that one of this proteins is apoprotein A-I, and the other one is probably a product of limited proteolysis of apoprotein A-I.
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