Biopolym. Cell. 1991; 7(4):97-103.
Cell Biology
Chloroplast DNA topoisomerase I from pea leaves
1Sitailo L. A.
- Institute of Cell Biology and Genetic Engineering, Academy of Sciences of the Ukrainian SSR
Kiev, USSR
Abstract
Chloroplasts of pea leaves were isolated by step gradient isoosmotic percoll, chloroplast DNA-topoisomerase. was isolated from them by fractionation in two-phase system ammonium sulfate/polyethylenglycol-6000 with the following purification by using column chromatography. The some properties of DNA-topoisomerase was searched. The dependence of relax activity of the enzyme from Mg2+ ions, its blocking by ethydium bromide, the absence of relax activity stimulation by polyamines: spermidine, spermin, cadaverine and the character of distribution of the topoisomers in the gels allow us to characterise the topoisomerase as prokaryotic type I.
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References
[1]
Botchan P, Wang JC, Echols H. Effect of circularity and superhelicity on transcription from bacteriophagelambda DNA. Proc Natl Acad Sci U S A. 1973;70(11):3077-81.
[2]
Champoux JJ, Been MD. Topoisomerases and the swivel problem. Mechanistic studies of DNA replication and genetic recombination. New York : Acad, press, 1980:809-815.
[3]
Maxwell A, Gellert M. Mechanistic aspects of DNA topoisomerases. Adv Protein Chem. 1986;38:69-107.
[4]
Wang JC. Type 1 DNA topoisomerases. The Enzymes. 1981; 14(1):331-44.
[5]
Thrash C, Voelkel K, DiNardo S, Sternglanz R. Identification of Saccharomyces cerevisiae mutants deficient in DNA topoisomerase I activity. J Biol Chem. 1984;259(3):1375-7.
[6]
Uemura T, Yanagida M. Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization. EMBO J. 1984;3(8):1737-44.
[7]
Maul GG, French BT, van Venrooij WJ, Jimenez SA. Topoisomerase I identified by scleroderma 70 antisera: enrichment of topoisomerase I at the centromere in mouse mitotic cells before anaphase. Proc Natl Acad Sci U S A. 1986;83(14):5145-9.
[8]
Yang L, Wold MS, Li JJ, Kelly TJ, Liu LF. Roles of DNA topoisomerases in simian virus 40 DNA replication in vitro. Proc Natl Acad Sci U S A. 1987;84(4):950-4.
[9]
McCoubrey WK Jr, Champoux JJ. The role of single-strand breaks in the catenation reaction catalyzed by the rat type I topoisomerase. J Biol Chem. 1986;261(11):5130-7.
[10]
Brill SJ, DiNardo S, Voelkel-Meiman K, Sternglanz R. Need for DNA topoisomerase activity as a swivel for DNA replication for transcription of ribosomal RNA. Nature. 1987 Mar 26-Apr 1;326(6111):414-6. Apr 23-29;326(6115):812.
[11]
Holm C, Goto T, Wang JC, Botstein D. DNA topoisomerase II is required at the time of mitosis in yeast. Cell. 1985;41(2):553-63.
[12]
Ross WE. DNA topoisomerases as targets for cancer therapy. Biochem Pharmacol. 1985;34(24):4191-5.
[13]
Kjeldsen E, Bonven BJ, Andoh T, Ishii K, Okada K, Bolund L, Westergaard O. Characterization of a camptothecin-resistant human DNA topoisomerase I. J Biol Chem. 1988;263(8):3912-6.
[14]
Pommier Y, Schwartz RE, Zwelling LA, Kohn KW. Effects of DNA intercalating agents on topoisomerase II induced DNA strand cleavage in isolated mammalian cell nuclei. Biochemistry. 1985;24(23):6406-10.
[15]
Tewey KM, Chen GL, Nelson EM, Liu LF. Intercalative antitumor drugs interfere with the breakage-reunion reaction of mammalian DNA topoisomerase II. J Biol Chem. 1984;259(14):9182-7.
[16]
Thompson RJ, Mosig G. An ATP-dependent supercoiling topoisomerase of Chlamydomonas reinhardtii affects accumulation of specific chloroplast transcripts. Nucleic Acids Res. 1985;13(3):873-91.
[17]
Dynan WS, Jendrisak JJ, Hager DA, Burgess RR. Purification and characterization of wheat germ DNA topoisomerase I (nicking-closing enzyme). J Biol Chem. 1981;256(11):5860-5.
[18]
Fukata H, Ohgami K, Fukasawa H. Isolation and characterization of DNA topoisomerase II from cauliflower inflorescences. Plant Mol Biol. 1986;6(3):137-44.
[19]
Lam E, Chua NH. Chloroplast DNA gyrase and in vitro regulation of transcription by template topology and novobiocin. Plant Mol Biol. 1987;8(5):415-24.
[20]
Siedlecki J, Zimmermann W, Weissbach A. Characterization of a prokaryotic topoisomerase I activity in chloroplast extracts from spinach. Nucleic Acids Res. 1983;11(5):1523-36.
[21]
Orozco EM Jr, Mullet JE, Hanley-Bowdoin L, Chua NH. In vitro transcription of chloroplast protein genes. Methods Enzymol. 1986;118:232-53.
[22]
Montecucco A, Ciarrocchi G. AMP-dependent DNA relaxation catalyzed by DNA ligase occurs by a nicking-closing mechanism. Nucleic Acids Res. 1988;16(15):7369-81.
[23]
Nielsen BL, Tewari KK. Pea chloroplast topoisomerase I: purification, characterization, and role in replication. Plant Mol Biol. 1988;11(1):3-14.
[24]
Karpenchuk KG, Rudenko GN. Nuclear DNA topoisomerase from the Zea mays embryos. Biopolym Cell. 1987; 3(2):77-82.
