Biopolym. Cell. 2010; 26(5):351-359.
Reviews
Nucleosome conformational flexibility in experiments with single chromatin fibers
1Sivolob A. V.
- Taras Shevchenko National University of Kyiv
64, Volodymyrska Str., Kyiv, Ukraine, 01601
Abstract
Studies on the chromatin nucleosome organization play an ever increasing role in our comprehension of mechanisms of the gene activity regulation. This minireview describes the results on the nucleosome conformational flexibility, which were obtained using magnetic tweezers to apply torsion to oligonucleosome fibers reconstituted on single DNA molecules. Such an approach revealed a new structural form of the nucleosome, the reversome, in which DNA is wrapped in a right-handed superhelix around a distorted histone octamer. Molecular mechanisms of the nucleosome structural flexibility and its biological relevance are discussed.
Keywords: nucleosome, DNA supercoiling, chromatin fiber, conformational flexibility
Full text: (PDF, in English) (PDF, in Russian)
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