Biopolym. Cell. 2008; 24(2):135-141.
Molecular and Cell Biotechnologies
Formaldehyde conductometric biosensor based on the recombinant formaldehyde dehydrogenase from Hansenula polymorpha yeast
1Sosovskaya O. F., 2Pavlishko G. N., 2Paryzhak S. Ya., 2Gonchar M. V., 1Korpan Ya. I.
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 - Institute of Cell Biology, NAS of Ukraine
14/16, Drahomanov Str., Lviv, Ukraine, 79005
Abstract
Gold interdigitated planar electrodes and NAD+- and glutathione-dependent formaldehyde dehydrogenase, isolated from the recombinant strain Tf 11-6 of the thermotolerant yeast H. polymorpha, were used for development of formaldehyde-sensitive conductometric biosensor. Novel approach to the preparation of sensor bioelement, including immobilisation of low-molecular cofactors (NAD+ and glutathione) in bioselective layer as well as allowing multiple assays without addition of the cofactors to the analyzed sample, was proposed. Dependence of biosensor response on analyte concentration, pH value, and buffer concentration was investigated using model samples. Selectivity, operational and storage stabilities of the developed sensor were studied. A linear detection range for formaldehyde was shown to be 1–100 mM.
Keywords: conductometric biosensor, Hansenula polymorpha, NAD+- and glutathione-dependent formaldehyde dehydrogenase, formaldehyde
Full text: (PDF, in English) (PDF, in Ukrainian)
References
[1]
Nikitina O., Shleev S., Gayda G., Demkiv O., Gonchar M., Gorton L., Csoregi E., Nistor M. Bi-enzyme biosensor based on NAD+- and glutathione-dependent recombinant formaldehyde dehydrogenase and diaphorase for formaldehyde assay. Sensors and Actuators. 2007; 125(1):1–9.
[2]
Kawamura K., Kerman K., Fujihara M., Nagatani N., Hashiba T., Tamiya E. Development of a novel hand-held formaldehyde gas sensor for the rapid detection of sick building syndrome. Sensors and Actuators. 2005; 105(2):495–501.
[3]
Ngamchana S., Surareungchai W. Sub-millimolar determination of formalin by pulsed amperometric detection. Analyt. Chim. Acta. 2004; 510(2):195–201.
[4]
Herschkovitz Y., Eshkenazi I., Campbell C. E., Rishpon J. An electrochemical biosensor for formaldehyde. J. Electroanalyt. Chem. 2000; 491(1-2):182–187.
[5]
Sibirnyi VA, Gonchar MV, Riabova OB, Ma?dan MM. Modern methods for formaldehyde, methanol and ethanol analysis. Mikrobiol Z. 2005;67(4):85-110.
[6]
Khlupova M, Kuznetsov B, Demkiv O, Gonchar M, Cs?regi E, Shleev S. Intact and permeabilized cells of the yeast Hansenula polymorpha as bioselective elements for amperometric assay of formaldehyde. Talanta. 2007;71(2):934-40.
[7]
Kataky R, Bryce MR, Goldenberg L, Hayes S, Nowak A. A biosensor for monitoring formaldehyde using a new lipophilic tetrathiafulvalene-tetracyanoquinodimethane salt and a polyurethane membrane. Talanta. 2002;56(3):451-8.
[8]
Korpan YI, Gonchar MV, Sibirny AA, Martelet C, El'skaya AV, Gibson TD, Soldatkin AP. Development of highly selective and stable potentiometric sensors for formaldehyde determination. Biosens Bioelectron. 2000;15(1-2):77-83.
[9]
Dzyadevych S. V., Arkhypova V. N., Korpan Y. I., El'skaya A. V., Soldatkin A. P., Jaffrezic-Renault N., Martelet C. Conductometric formaldehyde sensitive biosensor with specifically adapted analytical characteristics. Analyt. Chim. Acta. 2001; 445(1):47–55.
[10]
Soldatkin O. O., Sosovskaya O. F., Benilova I. V., Gonchar M. V., Korpan Y. I. Enzymatic conductometric sensor for formaldehyde detection in model samples. Biopolym. Cell. 2005; 21(5):425-432
[11]
Bunde RL, Jarvi EJ, Rosentreter JJ. A piezoelectric method for monitoring formaldehyde induced crosslink formation between poly-lysine and poly-deoxyguanosine. Talanta. 2000;51(1):159-71.
[12]
Demkiv O. M., Paryzhak S. Ya., Gayda G. Z., Sibirny V. A., Gonchar M. V. Formaldehyde dehydrogenase from recombinant yeast Hansenula polymorpha: isolation and bioanalytical application. FEMS Yeast Res. 2007; 7(7):1153–9.
[13]
Demkiv O. M., Paryzhak S. Ya., Krasovs'ka E. S., Stasyk O. V., Gayda G. Z., Sibirny A. A., Gonchar M. V. Construction of methylotrophic yeast Hansenula polymorpha strains over-producing formaldehyde dehydrogenase. Biopolym. Cell. 2005; 21(6):525-530
[14]
Shul'ga AA, Soldatkin AP, El'skaya AV, Dzyadevich SV, Patskovsky SV, Strikha VI. Thin-film conductometric biosensors for glucose and urea determination. Biosens Bioelectron. 1994;9(3):217-23.
[15]
Arkhypova V. M., Bereghetskyy A. L., Shul'ga O. A., Chovelon J.-M., Soldatkin A. P., Dzyadevych S. V. Investigation and optimization of conductometric transducers based on planar technology. Sensor Electron. Microsystem Technol. 2005; 2:48–54.
[16]
Ben Ali M, Korpan Y, Gonchar M, El'skaya A, Maaref MA, Jaffrezic-Renault N, Martelet C. Formaldehyde assay by capacitance versus voltage and impedance measurements using bi-layer bio-recognition membrane. Biosens Bioelectron. 2006;22(5):575-81.
[17]
Ben Ali M, Gonchar M, Gayda G, Paryzhak S, Maaref MA, Jaffrezic-Renault N, Korpan Y. Formaldehyde-sensitive sensor based on recombinant formaldehyde dehydrogenase using capacitance versus voltage measurements. Biosens Bioelectron. 2007;22(12):2790-5.
