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Biopolym. Cell. 2007; 23(2):137-142.
http://dx.doi.org/10.7124/bc.000760
Bioorganic Chemistry
Synthesis of 3'- and 3',5'-modified oligonucleotides on functionalized silica Silochrom-2
1Dubey I. Ya., 1Dubey L. V., 2Fedoryak D. M.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine
    1, Murmans'ka Str., Kyiv, Ukraine, 02094

Abstract

The preparation of 3'-mono- and 3', 5' -bis-labeled oligonucleotides on a new fluorescein-functionalized polymer support based on Silochrom-2 microspherical silica is described. Oligonucleotide (dT)15 containing a dye residue at 3'-end was synthesized on this support by solid-phase phosphoramidite method. Post-synthetic 5'-modification of 3'-conjugate allowed obtaining double dye-labeled oligomer. In the future it will be used for the synthesis of fluorescent probe for nucleic acids detection.
Keywords: oligonucleotide conjugates, fluorescent labels, solidphase synthesis, functionalization
Full text: (PDF, in English) (PDF, in Ukrainian)

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