Biopolym. Cell. 1997; 13(1):18-21.
Structure and Function of Biopolymers
The use of polyamines for creation of effective reconstructed protein synthesizing system from rabbit liver
1Palchevskii S. S.
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Abstract
A cell-free reconstructed protein-synthesizing system from rabbit liver has been developed. The system contains highly purified homologous components of translation apparatus and is characterized by a sufficient rate of polypeptide synthesis. The use of spermine and spermidine has allowed to optimize the activity of the system at physiological concentration of Mg2+.
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